| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0001 | RNA-Seq, substrate binding assay, enzyme activity assay, mass spectrometry | beta-mannan | Roseburia intestinalis | 30796211 The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y. |
2019 Feb 22 | degradation | 15 | 7 | GH1, CE2, GH130, GH130, GH36, GH113 |
| PUL0004 | enzyme activity assay, substrate binding assay | glucose, cellobiose, maltose | uncultured bacterium | 26827771 A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase. Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15. |
2016 Mar | degradation | 2 | 1 | GH1 |
| PUL0005 | enzyme activity assay, crystallization | beta-glucan, sophorose, laminaribiose | Listeria innocua | 26886583 Functional and Structural Analysis of a beta-Glucosidase Involved in beta-1,2-Glucan Metabolism in Listeria innocua. PLoS One. 2016 Feb 17;11(2):e0148870. doi: 10.1371/journal.pone.0148870. eCollection 2016. |
2016 | degradation | 2 | 1 | GH94, GH3 |
| PUL0024 | enzyme activity assay, qPCR, substrate binding assay | kestose | uncultured bacterium | 31915220 Harvesting of Prebiotic Fructooligosaccharides by Nonbeneficial Human Gut Bacteria. mSphere. 2020 Jan 8;5(1):e00771-19. doi: 10.1128/mSphere.00771-19. |
2020 Jan 8 | degradation | 12 | 1 | GH32 |
| PUL0025 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 32093600 Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549. Biochemistry (Mosc). 2020 Feb;85(2):241-247. doi: 10.1134/S000629792002011X. |
2020 Feb | biosynthesis | 18 | 4 | GT2, GT4, GT0, GT2 |
| PUL0046 | NMR, sequence homology analysis | O-antigen | Escherichia coli | 29738687 O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104. Biochemistry (Mosc). 2018 May;83(5):534-541. doi: 10.1134/S0006297918050061. |
2018 May | biosynthesis | 16 | 5 | GT2 |
| PUL0047 | sugar utilization assay, NMR, sequence homology analysis | O-antigen | Escherichia albertii | 31622726 Escherichia albertii EA046 (O9) harbors two polysaccharide gene clusters for synthesis of the O-antigen by the Wzx/Wzy-dependent pathway and a mannan shared by Escherichia coli O8 by the Wzm/Wzt-dependent pathway. Int J Biol Macromol. 2020 Jan 1;142:609-614. doi: 10.1016/j.ijbiomac.2019.09.135. Epub 2019 Oct 14. |
2020 Jan 1 | biosynthesis | 25 | 8 | GT4, GT4 |
| PUL0057 | NMR, sequence homology analysis | O-antigen | Escherichia coli | 29787897 Structural and genetic relatedness of the O-antigens of Escherichia coli O50 and O2. Carbohydr Res. 2018 Jul 15;464:8-11. doi: 10.1016/j.carres.2018.05.001. Epub 2018 May 7. |
2018 Jul 15 | biosynthesis | 13 | 5 | GT4, GT4 |
| PUL0059 | NMR, sequence homology analysis | O-antigen | Escherichia coli | 29787897 Structural and genetic relatedness of the O-antigens of Escherichia coli O50 and O2. Carbohydr Res. 2018 Jul 15;464:8-11. doi: 10.1016/j.carres.2018.05.001. Epub 2018 May 7. |
2018 Jul 15 | biosynthesis | 13 | 3 | GT2 |
| PUL0060 | NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 31421354 Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8. |
2019 Oct 1 | biosynthesis | 24 | 4 | GT2, GT2, GT4, GT2 |
| PUL0061 | NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 31421354 Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8. |
2019 Oct 1 | biosynthesis | 35 | 4 | GT2, GT2, GT4, GT2, GT4, CE4 |
| PUL0062 | NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 31421354 Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8. |
2019 Oct 1 | biosynthesis | 19 | 4 | GT2, GT2, GT4, GT2 |
| PUL0069 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 22 | 2 | GT0, GT2 |
| PUL0070 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 36 | 3 | GT52, GT0, GT14, GT2, GT25, GT4, CE4 |
| PUL0071 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 38 | 4 | GT0, GT14, GT2, GT25, GT4, CE4 |
| PUL0072 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 22 | 2 | GT2, GT0 |
| PUL0073 | sugar utilization assay, NMR, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii | 30664967 Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18. |
2019 May 1 | biosynthesis | 22 | 2 | GT2 |
| PUL0090 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia coli | 31220629 Structure elucidation and gene cluster characterization of the O-antigen of Yersinia kristensenii capital ES, Cyrillic-134. Carbohydr Res. 2019 Jul 15;481:9-15. doi: 10.1016/j.carres.2019.06.001. Epub 2019 Jun 6. |
2019 Jul 15 | biosynthesis | 12 | 4 | GT4, GT4, GT4 |
| PUL0099 | RNA-Seq, substrate binding assay, enzyme activity assay, mass spectrometry | beta-mannan | Roseburia intestinalis | 30796211 The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y. |
2019 Feb 22 | degradation | 3 | 2 | CBM27, GH26, CBM23, GH3, GH3 |
| PUL0125 | affinity gel electrophoresis, substrate binding assay, RNA-Seq | beta-glucan | Bacteroides ovatus | 31062073, 32801182 Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Mol Life Sci. 2019 Nov;76(21):4319-4340. doi: 10.1007/s00018-019-03115-3. Epub 2019 May 6. Appl Environ Microbiol. 2020 Oct 1;86(20):e01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1. |
2019 Nov,2020 Oct 1 | degradation | 6 | 2 | GH16, GH3 |
| PUL0130 | NMR, mass spectrometry | O-antigen | Yersinia rohdei | 30385338 Structure and gene cluster of the O-polysaccharide of Yersinia rohdei H274-36/78. Int J Biol Macromol. 2019 Feb 1;122:555-561. doi: 10.1016/j.ijbiomac.2018.10.189. Epub 2018 Oct 29. |
2019 Feb 1 | biosynthesis | 11 | 3 | GT4, GT4 |
| PUL0135 | enzyme activity assay, substrate binding assay | pectin | Pseudoalteromonas sp. | 30341080 Biochemical Reconstruction of a Metabolic Pathway from a Marine Bacterium Reveals Its Mechanism of Pectin Depolymerization. Appl Environ Microbiol. 2018 Dec 13;85(1):e02114-18. doi: 10.1128/AEM.02114-18. Print 2019 Jan 1. |
2019 Jan 1 | degradation | 21 | 8 | CE12, CE4, GH28, GH105, GH105, GH43, GH43_10, CE8, PL1_2 |
| PUL0160 | mass spectrometry, sequence homology analysis | alpha-mannan | Salegentibacter sp. Hel_I_6 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 33 | 10 | GH92, GH92, GH43, GH43_34, GH125, CBM32, GH92, GH92, GH92, GH76, GH2, GH92 |
| PUL0161 | mass spectrometry, sequence homology analysis | alpha-mannan | Bacteroides thetaiotaomicron | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 26 | 6 | GH97, GH67, GH76, GH92, GH76, GH125 |
| PUL0162 | mass spectrometry, sequence homology analysis | alpha-mannan | Bacteroides thetaiotaomicron | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 13 | 2 | GH92, GH99 |
| PUL0163 | mass spectrometry, sequence homology analysis | alpha-mannan | Bacteroides thetaiotaomicron | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 21 | 7 | GH92, GH38, CBM32, GT32, GT32, GH130, GH125, GH76, GH92, GH76 |
| PUL0164 | mass spectrometry, sequence homology analysis, differential gene expression | beta-mannan | Leeuwenhoekiella sp. MAR_2009_132 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 19 | 9 | CE2, GH3, GH5_7, GH26, GH130, GH26, GH5_2, GH5, GH27, GH9, GH26 |
| PUL0165 | mass spectrometry, sequence homology analysis, differential gene expression | beta-mannan | Salegentibacter sp. Hel_I_6 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 17 | 6 | GH9, GH27, GH5, GH5_2, GH26, GH130, GH26 |
| PUL0167 | mass spectrometry, sequence homology analysis | beta-mannan | Bacteroides ovatus | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 12 | 4 | GH130, GH36, GH26, GH26 |
| PUL0172 | gene deletion mutant and growth assay, NMR, sugar utilization assay | O-antigen | Franconibacter pulveris | 27166227 O antigen of FranconibacterpulverisG3872 (O1) is a 4-deoxy-d-arabino-hexose-containing polysaccharide synthesized by the ABC-transporter-dependent pathway. Microbiology (Reading). 2016 Jul;162(7):1103-1113. doi: 10.1099/mic.0.000307. Epub 2016 May 10. |
2016 Jul | biosynthesis | 9 | 3 | GT2, GT2 |
| PUL0173 | sequence homology analysis, NMR, sugar utilization assay | O-antigen | Escherichia coli | 27177202 Structure and gene cluster of the O-antigen of Escherichia coli O156 containing a pyruvic acid acetal. Carbohydr Res. 2016 Jul 22;430:24-28. doi: 10.1016/j.carres.2016.04.025. Epub 2016 Apr 29. |
2016 Jul 22 | biosynthesis | 20 | 4 | GT4, GT4, GT2 |
| PUL0177 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 27293097 Structure and Biosynthesis Gene Cluster of the O-Antigen of Escherichia coli O12. Biochemistry (Mosc). 2016 Apr;81(4):401-6. doi: 10.1134/S0006297916040106. |
2016 Apr | biosynthesis | 15 | 4 | GT4 |
| PUL0181 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 27083849 Structure of the beta-l-fucopyranosyl phosphate-containing O-specific polysaccharide of Escherichia coli O84. Int J Biol Macromol. 2016 Jul;88:578-85. doi: 10.1016/j.ijbiomac.2016.04.025. Epub 2016 Apr 13. |
2016 Jul | biosynthesis | 19 | 5 | GT4, GT2 |
| PUL0182 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 27058293 Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100. Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29. |
2016 May 13 | biosynthesis | 12 | 3 | GT2 |
| PUL0183 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 27058293 Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100. Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29. |
2016 May 13 | biosynthesis | 15 | 3 | GT2 |
| PUL0184 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 27058293 Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100. Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29. |
2016 May 13 | biosynthesis | 20 | 3 | GT4 |
| PUL0188 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 26582605 Structure and gene cluster of the O-antigen of Escherichia coli O165 containing 5-N-acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid. Glycobiology. 2016 Apr;26(4):335-42. doi: 10.1093/glycob/cwv106. Epub 2015 Nov 17. |
2016 Apr | biosynthesis | 10 | 3 | GT0, GT2 |
| PUL0198 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 26706815 Structure and gene cluster of the o-antigen of Escherichia coli o96. Carbohydr Res. 2016 Feb;420:1-5. doi: 10.1016/j.carres.2015.11.005. Epub 2015 Nov 23. |
2016 Feb | biosynthesis | 13 | 6 | GT2, GT2 |
| PUL0200 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 26451883 Structure and genetics of biosynthesis of the glycosyl phosphate-containing O-polysaccharide of Escherichia coli O160. Carbohydr Res. 2015 Nov 19;417:89-93. doi: 10.1016/j.carres.2015.09.007. Epub 2015 Sep 16. |
2015 Nov 19 | biosynthesis | 13 | 4 | GT2, GT2 |
| PUL0201 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 26382081 Structure elucidation and biosynthesis gene cluster organization of the O-antigen of Escherichia coli O170. Carbohydr Res. 2015 Nov 19;417:11-4. doi: 10.1016/j.carres.2015.08.013. Epub 2015 Aug 28. |
2015 Nov 19 | biosynthesis | 13 | 4 | GT2 |
| PUL0202 | NMR, sugar utilization assay | O-antigen | Escherichia coli | 26342864 Structure and gene cluster of the O-antigen of Escherichia coli O43. Carbohydr Res. 2015 Oct 30;416:32-6. doi: 10.1016/j.carres.2015.08.008. Epub 2015 Aug 18. |
2015 Oct 30 | biosynthesis | 19 | 4 | GT4, GT2 |
| PUL0203 | qPCR, thin layer chromatography, substrate binding assay | exopolysaccharide | Bacteroides thetaiotaomicron | 25841008 Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. |
2015 Jun 15 | degradation | 7 | 3 | GH32, GH32, GH32 |
| PUL0204 | qPCR, thin layer chromatography, substrate binding assay | exopolysaccharide | Bacteroides thetaiotaomicron | 25841008 Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. |
2015 Jun 15 | degradation | 7 | 3 | GH97 |
| PUL0205 | qPCR, thin layer chromatography, substrate binding assay | exopolysaccharide | Bacteroides thetaiotaomicron | 25841008 Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. |
2015 Jun 15 | degradation | 6 | 3 | GH31, GH31, GH66 |
| PUL0211 | enzyme activity assay, gene deletion mutant and growth assay, thin layer chromatography | N-glycan | Xanthomonas campestris pv. campestris | 25586188, 25205095 The N-Glycan cluster from Xanthomonas campestris pv. campestris: a toolbox for sequential plant N-glycan processing. The plant pathogen Xanthomonas campestris pv. campestris exploits N-acetylglucosamine during infection. J Biol Chem. 2015 Mar 6;290(10):6022-36. doi: 10.1074/jbc.M114.624593. Epub 2015 Jan 13. mBio. 2014 Sep 9;5(5):e01527-14. doi: 10.1128/mBio.01527-14. |
2015 Mar 6,2014 Sep 9 | degradation | 9 | 6 | GH29, GH18, GH20, GH2, GH3, GH125, GH92, GH35 |
| PUL0220 | mass spectrometry, target decoy database analysis | laminarin | Polaribacter sp. Hel1_33_49 | 25478683 Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom. ISME J. 2015 Jun;9(6):1410-22. doi: 10.1038/ismej.2014.225. Epub 2014 Dec 5. |
2015 Jun | degradation | 11 | 5 | GH30, GH30_1, GH17, GH3, GH149, GH16 |
| PUL0227 | enzyme activity assay, substrate binding assay | xylan | Caldanaerobius polysaccharolyticus | 22918832 Biochemical and structural insights into xylan utilization by the thermophilic bacterium Caldanaerobius polysaccharolyticus. J Biol Chem. 2012 Oct 12;287(42):34946-34960. doi: 10.1074/jbc.M112.391532. Epub 2012 Aug 22. |
2012 Oct 12 | degradation | 12 | 1 | GH67, GH3 |
| PUL0228 | qRT-PCR, enzyme activity assay, high performance anion exchange chromatography | arabinan | Bacteroides thetaiotaomicron | 22686399, 21339299 Prioritization of a plant polysaccharide over a mucus carbohydrate is enforced by a Bacteroides hybrid two-component system. The structure and function of an arabinan-specific alpha-1,2-arabinofuranosidase identified from screening the activities of bacterial GH43 glycoside hydrolases. Mol Microbiol. 2012 Aug;85(3):478-91. doi: 10.1111/j.1365-2958.2012.08123.x. Epub 2012 Jul 5. J Biol Chem. 2011 Apr 29;286(17):15483-95. doi: 10.1074/jbc.M110.215962. Epub 2011 Feb 21. |
2012 Aug,2011 Apr 29 | degradation | 10 | 4 | GH43, GH43_4, GH43_4, GH51, GH43_29 |
| PUL0230 | RT-PCR, enzyme activity assay, clone, enzyme kinetic analysis, thin layer chromatography, crystallization | maltooligosaccharide | Lactobacillus acidophilus | 22685275, 32444471 Enzymology and structure of the GH13_31 glucan 1,6-alpha-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM. An 1,4-alpha-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus. J Bacteriol. 2012 Aug;194(16):4249-59. doi: 10.1128/JB.00622-12. Epub 2012 Jun 8. Appl Environ Microbiol. 2020 Jul 20;86(15):e00661-20. doi: 10.1128/AEM.00661-20. Print 2020 Jul 20. |
2012 Aug,2020 Jul 20 | degradation | 12 | 4 | GH65, CBM34, GH13, GH13_20 |
| PUL0234 | proteome fractionation, mass spectrometry, target decoy database analysis | laminarin | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 7 | 3 | GH16, GH3, GH16 |
| PUL0235 | proteome fractionation, mass spectrometry, target decoy database analysis | alginate | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 20 | 7 | PL7, PL7_5, PL6_1, PL6, PL7, PL7_5, PL17, PL17_2, PL7, PL7 |
| PUL0236 | proteome fractionation, mass spectrometry, target decoy database analysis | alpha-glucan | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 13 | 4 | GH13, GH13, GH13_36, GH65, GH13_7 |
| PUL0244 | gene deletion mutant and growth assay, complementation study, substrate binding assay | sialic acid | Tannerella forsythia | 24351045 Structural and functional characterization of NanU, a novel high-affinity sialic acid-inducible binding protein of oral and gut-dwelling Bacteroidetes species. Biochem J. 2014 Mar 15;458(3):499-511. doi: 10.1042/BJ20131415. |
2014 Mar 15 | degradation | 9 | 2 | GH20, GH33 |
| PUL0283 | microarray, high performance anion exchange chromatography, liquid chromatography and mass spectrometry | galactooligosaccharide | Bifidobacterium breve | 23199239 Transcriptional and functional characterization of genetic elements involved in galacto-oligosaccharide utilization by Bifidobacterium breve UCC2003. Microb Biotechnol. 2013 Jan;6(1):67-79. doi: 10.1111/1751-7915.12011. Epub 2012 Dec 2. |
2013 Jan | degradation | 6 | 1 | GH42, GH53, CBM61 |
| PUL0284 | microarray, high performance anion exchange chromatography, liquid chromatography and mass spectrometry | galactooligosaccharide | Bifidobacterium breve | 23199239 Transcriptional and functional characterization of genetic elements involved in galacto-oligosaccharide utilization by Bifidobacterium breve UCC2003. Microb Biotechnol. 2013 Jan;6(1):67-79. doi: 10.1111/1751-7915.12011. Epub 2012 Dec 2. |
2013 Jan | degradation | 3 | 1 | GH2 |
| PUL0285 | microarray, high performance anion exchange chromatography, liquid chromatography and mass spectrometry | galactooligosaccharide | Bifidobacterium breve | 23199239 Transcriptional and functional characterization of genetic elements involved in galacto-oligosaccharide utilization by Bifidobacterium breve UCC2003. Microb Biotechnol. 2013 Jan;6(1):67-79. doi: 10.1111/1751-7915.12011. Epub 2012 Dec 2. |
2013 Jan | degradation | 6 | 1 | GH42 |
| PUL0286 | NMR, mass spectrometry, sugar utilization assay | O-antigen | Providencia alcalifaciens | 23163869 Structural, serological, and genetic characterization of the O-antigen of Providencia alcalifaciens O40. FEMS Immunol Med Microbiol. 2012 Dec;66(3):382-92. doi: 10.1111/1574-695X.12002. |
2012 Dec | biosynthesis | 16 | 4 | GT2, GT4, GT2 |
| PUL0288 | acid hydrolysis, NMR, mass spectrometry, sugar utilization assay | capsule polysaccharide, heparosan | Escherichia coli | 22975275 Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12. Carbohydr Res. 2012 Oct 1;360:19-24. doi: 10.1016/j.carres.2012.07.013. Epub 2012 Jul 27. |
2012 Oct 1 | biosynthesis | 5 | 1 | GT2 |
| PUL0290 | NMR, mass spectrometry, sugar utilization assay | O-antigen | Yersinia intermedia | 29524727 Full structure and insight into the gene cluster of the O-specific polysaccharide of Yersinia intermedia H9-36/83 (O:17). Carbohydr Res. 2018 May 2;460:51-56. doi: 10.1016/j.carres.2018.02.014. Epub 2018 Feb 28. |
2018 May 2 | degradation | 12 | 4 | GT4, GT4 |
| PUL0301 | sugar utilization assay, NMR | O-antigen | Escherichia coli | 29309918 Structure elucidation of the O-specific polysaccharide by NMR spectroscopy and selective cleavage and genetic characterization of the O-antigen of Escherichia albertii O5. Carbohydr Res. 2018 Mar 2;457:25-31. doi: 10.1016/j.carres.2017.12.010. Epub 2018 Jan 3. |
2018 Mar 2 | biosynthesis | 10 | 3 | GT32, GT2, GT2 |
| PUL0308 | microarray, enzyme activity assay, high performance anion exchange chromatography, mass spectrometry, RNA-seq | beta-glucan | Bacteroides ovatus | 29020628, 22205877, 32801182 Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Rep. 2017 Oct 10;21(2):417-430. doi: 10.1016/j.celrep.2017.09.049. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Appl Environ Microbiol. 2020 Oct 1;86(20):e01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1. |
2017 Oct 10,2011 Dec,2020 Oct 1 | degradation | 5 | 3 | GH16, GH3 |
| PUL0309 | enzyme activity assay, substrate binding assay, isothermal titration calorimetry | arabinan | Caldanaerobius polysaccharolyticus | 28710263 Enzymatic Mechanism for Arabinan Degradation and Transport in the Thermophilic Bacterium Caldanaerobius polysaccharolyticus. Appl Environ Microbiol. 2017 Aug 31;83(18):e00794-17. doi: 10.1128/AEM.00794-17. Print 2017 Sep 15. |
2017 Sep 15 | degradation | 12 | 6 | GH43_4, GH43, GH127, GH51, GH27, GH51, GH146 |
| PUL0320 | liquid chromatography and mass spectrometry, mass spectrometry, target decoy database analysis | glucan | Caldicellulosiruptor bescii | 29475869, 29588665 Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses. The diversity and specificity of the extracellular proteome in the cellulolytic bacterium Caldicellulosiruptor bescii is driven by the nature of the cellulosic growth substrate. Appl Environ Microbiol. 2018 Apr 16;84(9):e02694-17. doi: 10.1128/AEM.02694-17. Print 2018 May 1. Biotechnol Biofuels. 2018 Mar 23;11:80. doi: 10.1186/s13068-018-1076-1. eCollection 2018. |
2018 May 1,2018 | degradation | 19 | 9 | CE12, PL11, CBM3, PL3_1, CBM66, PL3, PL9_1, PL9, CBM66, CBM22, GH10, GH48, CBM3, GH44, GH5, CBM3, GH5_8, GH48, GH74, CBM3, GT39, GH5, GH9, CBM3, GH5_8, GH5_1, CBM3, GH5_8, GH9, GH48, CBM3 |
| PUL0326 | gene deletion mutant and growth assay, enzyme activity assay, thin layer chromatography | beta-glucan | Bacteroides ovatus | 28461332 A Bacteroidetes locus dedicated to fungal 1,6-beta-glucan degradation: Unique substrate conformation drives specificity of the key endo-1,6-beta-glucanase. J Biol Chem. 2017 Jun 23;292(25):10639-10650. doi: 10.1074/jbc.M117.787606. Epub 2017 May 1. |
2017 Jun 23 | degradation | 13 | 2 | GH73 |
| PUL0330 | fosmid library screen, enzyme activity assay, thin layer chromatography | pectin | Gramella flava | 28261179 Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017. |
2017 | degradation | 28 | 11 | CE8, PL9_1, GH28, GH105, GH43_10, GH28, PL9_1, CE12, CE8, CE10, CE12, PL10_1 |
| PUL0332 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 19 | 9 | GH5, GH5_2, GH5_7, GH5, CE7, GH2, GH94, GH97, GH127, GH127 |
| PUL0333 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 22 | 3 | GH9, GH31, GH9 |
| PUL0334 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 23 | 5 | GH9, GH31, GH9, GH9, CE4 |
| PUL0335 | fosmid library screen, enzyme activity assay, thin layer chromatography | xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 31 | 4 | GH13, GH97, GH3, GH158, GH16 |
| PUL0336 | fosmid library screen, enzyme activity assay, thin layer chromatography | xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 25 | 3 | GH3, GH158, GH16 |
| PUL0337 | fosmid library screen, enzyme activity assay, thin layer chromatography | xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 29 | 3 | GH16, GH158, GH3 |
| PUL0338 | fosmid library screen, enzyme activity assay, thin layer chromatography | xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 34 | 4 | GH16, GH158, GH3, GH97 |
| PUL0339 | fosmid library screen, enzyme activity assay, thin layer chromatography | xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 24 | 4 | GH97, GH3, GH16, GH20 |
| PUL0340 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 37 | 1 | GH5_2, GH5 |
| PUL0341 | fosmid library screen, enzyme activity assay, thin layer chromatography | carboxymethylcellulose, xylan, beta-glucan, lichenan | uncultured bacterium | 28091525 A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248. |
2017 Jan 16 | degradation | 43 | 3 | GH32, GH91, GH5, GH5_2 |
| PUL0367 | mass spectrometry | galactooligosaccharide | Bifidobacterium longum | 18539808 Differential transcriptional response of Bifidobacterium longum to human milk, formula milk, and galactooligosaccharide. Appl Environ Microbiol. 2008 Aug;74(15):4686-94. doi: 10.1128/AEM.00122-08. Epub 2008 Jun 6. |
2008 Aug | degradation | 3 | 1 | GH42 |
| PUL0385 | ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high performance anion exchange chromatography | cellulose | Ruminiclostridium cellulolyticum | 20013800 Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. |
2010 Feb | degradation | 12 | 10 | GH48, GH8, GH9, CBM3, CBM4, GH9, CBM30, GH9, CBM3, GH9, CBM3, GH5_17, GH9, PL11_1, PL11, GH5_1 |
| PUL0386 | ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high performance anion exchange chromatography | hemicellulose | Ruminiclostridium cellulolyticum | 20013800 Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. |
2010 Feb | degradation | 11 | 11 | GH43, CBM6, GH43_16, GH10, CBM6, GH43_29, GH43, CBM6, CE1, CBM6, GH43_10, CBM6, GH62, CBM6, GH43_29, GH43, CBM6, GH146, CBM22, GH27, CBM6, GH59, CBM6, GH2, CBM6 |
| PUL0388 | sugar utilization assay, NMR | O-antigen | Salmonella enterica | 20501518 Structural and genetic characterization of the closely related O-antigens of Escherichia coli O85 and Salmonella enterica O17. Innate Immun. 2011 Apr;17(2):164-73. doi: 10.1177/1753425910369270. Epub 2010 May 25. |
2011 Apr | biosynthesis | 9 | 4 | GT4, GT4, GT2 |
| PUL0389 | sugar utilization assay, NMR | O-antigen | Escherichia coli | 20501518 Structural and genetic characterization of the closely related O-antigens of Escherichia coli O85 and Salmonella enterica O17. Innate Immun. 2011 Apr;17(2):164-73. doi: 10.1177/1753425910369270. Epub 2010 May 25. |
2011 Apr | biosynthesis | 8 | 4 | GT4, GT4, GT2 |
| PUL0406 | high performance anion exchange chromatography | beta-glucan | Coprothermobacter proteolyticus | 30315317 From proteins to polysaccharides: lifestyle and genetic evolution of Coprothermobacter proteolyticus. ISME J. 2019 Mar;13(3):603-617. doi: 10.1038/s41396-018-0290-y. Epub 2018 Oct 12. |
2019 Mar | degradation | 20 | 3 | GH16, GH3, GH18 |
| PUL0408 | enzyme activity assay, thin layer chromatography | beta-mannan | Bacteroides fragilis | 24217874 The mannobiose-forming exo-mannanase involved in a new mannan catabolic pathway in Bacteroides fragilis. Arch Microbiol. 2014 Jan;196(1):17-23. doi: 10.1007/s00203-013-0938-y. Epub 2013 Nov 12. |
2014 Jan | degradation | 4 | 2 | GH26, GH130 |
| PUL0414 | enzyme activity assay, thin layer chromatography | xylan | uncultured bacterium 35A20 | 30116044 Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite. ISME J. 2019 Jan;13(1):104-117. doi: 10.1038/s41396-018-0255-1. Epub 2018 Aug 16. |
2019 Jan | degradation | 28 | 7 | GH10, GH10 |
| PUL0431 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia coli | 27454490 Structure elucidation and gene cluster characterization of the O-antigen of Escherichia coli O80. Carbohydr Res. 2016 Sep 2;432:83-7. doi: 10.1016/j.carres.2016.07.011. Epub 2016 Jul 14. |
2016 Sep 2 | biosynthesis | 18 | 4 | GT26 |
| PUL0432 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia coli | 27645300 Structures and gene clusters of the O-specific polysaccharides of the lipopolysaccharides of Escherichia coli O69 and O146 containing glycolactilic acids: ether conjugates of D-GlcNAc and D-Glc with (R)- and (S)-lactic acid. Glycoconj J. 2017 Feb;34(1):71-84. doi: 10.1007/s10719-016-9730-y. Epub 2016 Sep 19. |
2017 Feb | biosynthesis | 11 | 3 | GT4, GT2 |
| PUL0433 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia coli | 27645300 Structures and gene clusters of the O-specific polysaccharides of the lipopolysaccharides of Escherichia coli O69 and O146 containing glycolactilic acids: ether conjugates of D-GlcNAc and D-Glc with (R)- and (S)-lactic acid. Glycoconj J. 2017 Feb;34(1):71-84. doi: 10.1007/s10719-016-9730-y. Epub 2016 Sep 19. |
2017 Feb | biosynthesis | 12 | 3 | GT2 |
| PUL0435 | mass spectrometry, high performance anion exchange chromatography | glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 13 | 4 | GH43, GH43_34, GH30, GH30_1, GH30, GH30_3, GH16 |
| PUL0436 | mass spectrometry, high performance anion exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 8 | 2 | GH65, GH13, CBM32 |
| PUL0437 | mass spectrometry, high performance anion exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 6 | 1 | GH51 |
| PUL0438 | mass spectrometry, high performance anion exchange chromatography | glucomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 7 | 3 | GH89, GH158 |
| PUL0439 | mass spectrometry, high performance anion exchange chromatography | glucomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 4 | 1 | GH33 |
| PUL0440 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 5 | 2 | GH2, GH76, CBM13 |
| PUL0441 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 6 | 2 | GH130, CBM13, GH43_26 |
| PUL0442 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 9 | 3 | CBM5, GH18, GH18, GH64, CBM6, GH16, CBM6 |
| PUL0443 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 9 | 3 | GH16, CBM6 |
| PUL0444 | mass spectrometry, high performance anion exchange chromatography | glucomannan, galactomannan, glucose | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 6 | 2 | GH31, GH19, CBM5 |
| PUL0445 | recombinant protein expression, thin layer chromatography, enzyme activity assay | alginate | Sphingomonas sp. | 10913091 Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate. J Bacteriol. 2000 Aug;182(16):4572-7. doi: 10.1128/JB.182.16.4572-4577.2000. |
2000 Aug | degradation | 8 | 2 | PL7, PL5, PL15_1 |
| PUL0446 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia albertii | 28494314 Structure and gene cluster of the O-antigen of Escherichia albertii O1 resembling the O-antigen of Pseudomonas aeruginosa O5. Carbohydr Res. 2017 Jun 29;446-447:28-31. doi: 10.1016/j.carres.2017.04.024. Epub 2017 May 2. |
2017 Jun 29 | biosynthesis | 17 | 2 | GT4 |
| PUL0447 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia albertii | 28672166 Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17. |
2017 Sep 8 | biosynthesis | 14 | 4 | GT4, GT4 |
| PUL0448 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia albertii | 28672166 Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17. |
2017 Sep 8 | biosynthesis | 19 | 4 | GT4 |
| PUL0449 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia albertii | 28672166 Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17. |
2017 Sep 8 | biosynthesis | 13 | 4 | GT4, GT2, GT4 |
| PUL0450 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia albertii | 28672166 Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17. |
2017 Sep 8 | biosynthesis | 14 | 3 | GT52, GT2, GT2 |
| PUL0451 | sequence homology analysis, sugar utilization assay, NMR, gas chromatography and mass spectrometry | O-antigen | Streptococcus pneumoniae | 28837839 Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F. Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18. |
2017 Oct 10 | biosynthesis | 18 | 5 | GT4 |
| PUL0452 | sequence homology analysis, sugar utilization assay, NMR, gas chromatography and mass spectrometry | O-antigen | Streptococcus pneumoniae | 28837839 Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F. Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18. |
2017 Oct 10 | biosynthesis | 18 | 5 | GT4, GT2 |
| PUL0453 | sequence homology analysis, sugar utilization assay, NMR, gas chromatography and mass spectrometry | O-antigen | Streptococcus pneumoniae | 28837839 Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F. Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18. |
2017 Oct 10 | biosynthesis | 18 | 5 | GT4 |
| PUL0457 | high performance anion exchange chromatography, enzyme activity assay, RNA-Seq | xylooligosaccharide | Lactobacillus rossiae | 27142164 Cloning, expression and characterization of a beta-D-xylosidase from Lactobacillus rossiae DSM 15814(T). Microb Cell Fact. 2016 May 3;15:72. doi: 10.1186/s12934-016-0473-z. |
2016 May 3 | degradation | 7 | 1 | GH43_11, GH43 |
| PUL0461 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Yersinia kristensenii | 31220629 Structure elucidation and gene cluster characterization of the O-antigen of Yersinia kristensenii capital ES, Cyrillic-134. Carbohydr Res. 2019 Jul 15;481:9-15. doi: 10.1016/j.carres.2019.06.001. Epub 2019 Jun 6. |
2019 Jul 15 | biosynthesis | 12 | 4 | GT4, GT4, GT4 |
| PUL0462 | sequence homology analysis, sugar utilization assay, NMR | O-antigen | Escherichia coli | 24607538 Structure elucidation and gene cluster annotation of the O-antigen of Escherichia coli O39; application of anhydrous trifluoroacetic acid for selective cleavage of glycosidic linkages. Carbohydr Res. 2014 Mar 31;388:30-6. doi: 10.1016/j.carres.2014.02.013. Epub 2014 Feb 18. |
2014 Mar 31 | biosynthesis | 14 | 4 | GT0, GT4, GT2 |
| PUL0542 | substrate binding assay | xylan | Geobacillus stearothermophilus | 10368143 The glucuronic acid utilization gene cluster from Bacillus stearothermophilus T-6. J Bacteriol. 1999 Jun;181(12):3695-704. doi: 10.1128/JB.181.12.3695-3704.1999. |
1999 Jun | degradation | 30 | 6 | CE4, GH52, GH10, GH67, GH39, GH43_11 |
| PUL0582 | NMR, microarray, enzyme activity assay, gene deletion mutant and growth assay | lactose | Lactococcus lactis | 22660716 A specific mutation in the promoter region of the silent cel cluster accounts for the appearance of lactose-utilizing Lactococcus lactis MG1363. Appl Environ Microbiol. 2012 Aug;78(16):5612-21. doi: 10.1128/AEM.00455-12. Epub 2012 Jun 1. |
2012 Aug | degradation | 5 | 1 | GH1 |
| PUL0589 | Western Blot, enzyme activity assay, thin layer chromatography | starch | Streptococcus mutans | 23930155 The malQ gene is essential for starch metabolism in Streptococcus mutans. J Oral Microbiol. 2013 Aug 6;5. doi: 10.3402/jom.v5i0.21285. Print 2013. |
2013 | degradation | 3 | 2 | GT35, GH77 |
| PUL0595 | enzyme activity assay, qPCR, crystallization | starch | [Eubacterium] rectale | 25388295 Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol Microbiol. 2015 Jan;95(2):209-30. doi: 10.1111/mmi.12859. Epub 2014 Dec 19. |
2015 Jan | degradation | 4 | 1 | CBM26, GH13_41, CBM82, GH13, CBM41, CBM83 |
| PUL0596 | enzyme activity assay, qPCR, crystallization | starch | [Eubacterium] rectale | 25388295 Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol Microbiol. 2015 Jan;95(2):209-30. doi: 10.1111/mmi.12859. Epub 2014 Dec 19. |
2015 Jan | degradation | 4 | 1 | GH13_36 |
| PUL0608 | enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry, thin layer chromatography, MALDI-TOF/MS | human milk oligosaccharide | Roseburia hominis DSM 16839 | 32620774 Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x. |
2020 Jul 3 | degradation | 9 | 2 | 3.2.1.140, GH136, 2.4.1.211, GH112 |
| PUL0609 | enzyme activity assay, clone and expression, liquid chromatography and mass spectrometry, thin layer chromatography, MALDI-TOF/MS | human milk oligosaccharide | Roseburia inulinivorans DSM 16841 | 32620774 Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x. |
2020 Jul 3 | degradation | 11 | 4 | GH112, 2.4.1.211, 3.2.1.63, GH95, 3.2.1.63, GH95, GH136, 3.2.1.- |
| PUL0626 | high performance anion exchange chromatography | arabinan | Lactobacillus crispatus DSM29598 | 33119797 Characterization of two extracellular arabinanases in Lactobacillus crispatus. Appl Microbiol Biotechnol. 2020 Dec;104(23):10091-10103. doi: 10.1007/s00253-020-10979-0. Epub 2020 Oct 29. |
2020 Dec | degradation | 24 | 8 | GH43, CBM54, GH43_4, GH2, GH43, GH43_4, GH127, GH51, GH43, CBM42, GH43_26, GH51, GH27, CBM51 |
| PUL0627 | sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii LUH5543 | 33159946 Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4. |
2021 Jan 1 | biosynthesis | 21 | 1 | GT2 |
| PUL0628 | sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii BAL_204 | 33159946 Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4. |
2021 Jan 1 | biosynthesis | 22 | 2 | GT2, GT2, GT2, GT2 |
| PUL0629 | sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis | capsule polysaccharide | Acinetobacter baumannii BAL_309 | 33159946 Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4. |
2021 Jan 1 | biosynthesis | 22 | 1 | GT2, GT2 |
| PUL0643 | enzyme activity assay, liquid chromatography, high performance anion exchange chromatography, qRT-PCR, crystallization | arabinoxylan | Bacteroides intestinalis DSM 17393 | 33469030 Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5. |
2021 Jan 19 | degradation | 12 | 7 | CBM48, CE1, GH43, GH43_7, CBM13, CBM48, CE1, CE6, CBM48, CE1, GH43_17, CBM6, GH3, GH43, CBM6, GH43_2 |
| PUL0644 | enzyme activity assay, liquid chromatography, high performance anion exchange chromatography, qRT-PCR, crystallization | arabinoxylan | Bacteroides cellulosilyticus DSM 14838 | 33469030 Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5. |
2021 Jan 19 | degradation | 10 | 6 | GH43, GH43_2, CBM6, GH43_17, GH10, CBM48, CE1, CE1, GH43, CBM13, GH43_7, CBM48, CE1 |
| PUL0645 | enzyme activity assay, liquid chromatography, high performance anion exchange chromatography, qRT-PCR, crystallization | arabinoxylan | Bacteroides oleiciplenus YIT 12058 | 33469030 Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat Commun. 2021 Jan 19;12(1):459. doi: 10.1038/s41467-020-20737-5. |
2021 Jan 19 | degradation | 10 | 5 | GH43, GH43_2, CBM6, GH3, CBM6, GH43_17, GH43_7, GH43, CBM13, CE1, CBM48 |
| PUL0646 | recombinant protein expression, crystallization, affinity gel electrophoresis, isothermal titration calorimetry | beta-glucan | Bacteroides fluxus YIT 12057 | 33587952 Distinct protein architectures mediate species-specific beta-glucan binding and metabolism in the human gut microbiota. J Biol Chem. 2021 Jan-Jun;296:100415. doi: 10.1016/j.jbc.2021.100415. Epub 2021 Feb 13. |
2021 Jan-Jun | degradation | 6 | 2 | GH3, CBM6, GH158 |
| PUL0648 | high performance anion exchange chromatography, substrate binding assay, thin layer chromatography, NMR, mass spectrometry, crystallization | glucuronoarabinoxylan | Dysgonomonas mossii DSM 22836 | 33667545 A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2. |
2021 Jan-Jun | degradation | 37 | 20 | CBM0, 3.2.1.8, CBM4, GH10, GH43_12, GH43, GH43, GH43_1, GH10, GH146, CBM48, CE1, CBM6, CBM36, GH8, 3.2.1.156, CE6, CBM2, GH43_29, GH43, CBM22, CBM42, CBM66, CBM6, CBM6, GH43_29, GH43, GH97, GH31, GH51, CBM2, GH43_29, GH43, CBM22, CBM42, CBM6, CBM48, CE1, GH10, GH43_10, GH43, GH115, GH10, GH43, GH43_1, GH67 |
| PUL0649 | Smith degradation, NMR | capsule polysaccharide | Acinetobacter baumannii B8300 | 33667610 A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300. Res Microbiol. 2021 Apr-May;172(3):103815. doi: 10.1016/j.resmic.2021.103815. Epub 2021 Mar 3. |
2021 Apr-May | biosynthesis | 22 | 1 | GT2, GT2 |
| PUL0650 | enzyme activity assay, high performance anion exchange chromatography, recombinant protein expression, NMR, gene deletion mutant and growth assay | arabinogalactan | Bifidobacterium longum JCM 7052 | 33674431 Novel 3-O-alpha-d-Galactosyl-alpha-l-Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum. Appl Environ Microbiol. 2021 Apr 27;87(10):e02690-20. doi: 10.1128/AEM.02690-20. Print 2021 Apr 27. |
2021 Apr 27 | degradation | 7 | 2 | GH36, GH39, CBM35 |
| PUL0651 | enzyme activity assay, NMR | agar | Gilvimarinus chinensis DSM 19667 | 33691998 Agarase cocktail from agar polysaccharide utilization loci converts homogenized Gelidium amansii into neoagarooligosaccharides. Food Chem. 2021 Aug 1;352:128685. doi: 10.1016/j.foodchem.2020.128685. Epub 2020 Nov 19. |
2021 Aug 1 | degradation | 63 | 14 | CBM6, GH16_16, GH16, GH50, CBM6, CBM13, CBM6, GH16_16, GH16, GH50, GH117, GH16_3, GH16, GH2, GH50, GH127, GH167, CBM2, CBM6, GH86, 3.2.1.81, GH50, 3.2.1.81, GH86 |
| PUL0652 | RNA-Seq, enzyme activity assay, thin layer chromatography, liquid chromatography, mass spectrometry | agar | Colwellia echini A3 | 33811026 A Novel Auxiliary Agarolytic Pathway Expands Metabolic Versatility in the Agar-Degrading Marine Bacterium Colwellia echini A3(T). Appl Environ Microbiol. 2021 May 26;87(12):e0023021. doi: 10.1128/AEM.00230-21. Epub 2021 May 26. |
2021 May 26 | degradation | 54 | 10 | CBM35, GH2, CBM6, GH96, CBM6, GH96, GH50, GH50, GH50, GH29, CBM13, GH50, GH86, GH117 |
| PUL0657 | recombinant protein expression, NMR | levoglucosan | Bacillus smithii S-2701M | 33208778 Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction. Sci Rep. 2020 Nov 18;10(1):20066. doi: 10.1038/s41598-020-77133-8. |
2020 Nov 18 | degradation | 8 | 2 | GH109, GH109 |
| PUL0660 | NMR, Smith degradation | capsule polysaccharide | Acinetobacter baumannii 48-1789 | 34073255 Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides. Int J Mol Sci. 2021 May 26;22(11):5641. doi: 10.3390/ijms22115641. |
2021 May 26 | biosynthesis | 21 | 2 | GT2, GT4 |
| PUL0661 | NMR, Smith degradation | capsule polysaccharide | Acinetobacter baumannii MAR-24 | 34073255 Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides. Int J Mol Sci. 2021 May 26;22(11):5641. doi: 10.3390/ijms22115641. |
2021 May 26 | biosynthesis | 22 | 2 | GT2, GT4 |
| PUL0662 | thin layer chromatography, liquid chromatography and mass spectrometry, qPCR, clone and expression | β-mannan | Phocaeicola dorei DSM 17855 | 34339781 BdPUL12 depolymerizes beta-mannan-like glycans into mannooligosaccharides and mannose, which serve as carbon sources for Bacteroides dorei and gut probiotics. Int J Biol Macromol. 2021 Sep 30;187:664-674. doi: 10.1016/j.ijbiomac.2021.07.172. Epub 2021 Jul 31. |
2021 Sep 30 | degradation | 9 | 4 | GH5_7, CE7, GH26, GH130 |
| PUL0663 | thin layer chromatography, clone and expression, recombinant protein expression | arabinogalactan protein | Bacteroides plebeius DSM17135 | 34340552 Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius. mBio. 2021 Aug 31;12(4):e0136821. doi: 10.1128/mBio.01368-21. Epub 2021 Aug 3. |
2021 Aug 31 | degradation | 7 | 4 | GH145, GH43_24, GH43_17, GH105 |
| PUL0664 | thin layer chromatography, clone and expression, recombinant protein expression | arabinogalactan protein | Bacteroides plebeius DSM17135 | 34340552 Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius. mBio. 2021 Aug 31;12(4):e0136821. doi: 10.1128/mBio.01368-21. Epub 2021 Aug 3. |
2021 Aug 31 | degradation | 17 | 8 | GH49, GH2, GH36, GH27, GH28, GH43, GH43, GH2 |
| PUL0669 | clone, high performance anion exchange chromatography, enzymatic product analysis | xylan | Bacteroides eggerthii 1_2_48FAA | 34480044 Characterization of a novel multidomain CE15-GH8 enzyme encoded by a polysaccharide utilization locus in the human gut bacterium Bacteroides eggerthii. Sci Rep. 2021 Sep 3;11(1):17662. doi: 10.1038/s41598-021-96659-z. |
2021 Sep 3 | degradation | 24 | 12 | CE15, GH8, GH95, GH43 |
| PUL0670 | NMR, Smith degradation, mass spectrometry | capsule polysaccharide | Acinetobacter baumannii KZ-1098 | 34537298 The K26 capsular polysaccharide from Acinetobacter baumannii KZ-1098: Structure and cleavage by a specific phage depolymerase. Int J Biol Macromol. 2021 Nov 30;191:182-191. doi: 10.1016/j.ijbiomac.2021.09.073. Epub 2021 Sep 16. |
2021 Nov 30 | biosynthesis | 24 | 2 | GT2, GT4 |
| PUL0672 | Smith degradation, NMR, mass spectrometry | capsule polysaccharide | Acinetobacter baumannii Ab-46-1632 | 34757131 Correlation of Acinetobacter baumannii K144 and K86 capsular polysaccharide structures with genes at the K locus reveals the involvement of a novel multifunctional rhamnosyltransferase for structural synthesis. Int J Biol Macromol. 2021 Dec 15;193(Pt B):1294-1300. doi: 10.1016/j.ijbiomac.2021.10.178. Epub 2021 Oct 30. |
2021 Dec 15 | biosynthesis | 20 | 4 | GT2, GT2, GT2, GT2 |
| PUL0673 | NMR, substrate binding assay, liquid chromatography and mass spectrometry | human milk oligosaccharide | Bifidobacterium pseudocatenulatum DSM20438 | 34757822 Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity. Appl Environ Microbiol. 2022 Jan 25;88(2):e0170721. doi: 10.1128/AEM.01707-21. Epub 2021 Nov 10. |
2022 Jan 25 | degradation | 8 | 1 | GH95 |
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